Hutsell T C, Stachelski S J
J Chromatogr. 1975 Mar 19;106(1):151-8. doi: 10.1016/s0021-9673(01)81057-8.
A rapid, precise and accurate assay for disopyramide and mono-N-dealkylated disopyramide concentrations in blood serum and urine is described. The method involves extraction of the drugs from a basic aqueous medium into chloroform, derivatization of the metabolite, purification of the extract and gas chromatographic analysis using an OV-17 liquid phase and flame ionization detection. Unique characteristics of the procedure, direct derivatization in the organic phase and the use of Florisil to separate the drugs from interfering materials, should be applicable to the analysis of other basic drugs in biological specimens.
本文描述了一种快速、精确且准确的血清和尿液中丙吡胺及单 -N- 去烷基丙吡胺浓度测定方法。该方法包括从碱性水性介质中用氯仿萃取药物、代谢物衍生化、提取物纯化以及使用 OV - 17 固定相和火焰离子化检测进行气相色谱分析。该方法的独特之处在于在有机相中直接衍生化以及使用弗罗里硅土从干扰物质中分离药物，应适用于生物样品中其他碱性药物的分析。
