[Characteristics of the NAD-dependent alpha-glycerolphosphate dehydrogenase isoenzymes from vertebrate and human muscles according to polyacrylamide gel electrophoretic and isoelectric focusing data].

Electrophoresis and isoelectric focusing in polyacrylamide gel were used to demonstrate the presence of 5 molecular forms of alpha-glycerophosphate dehydrogenase (E. C. I. I. I. 8, alpha-GDH) in the cytoplasm of some vertebrata skeletal muscles. Assay of alpha-GDH isozyme distribution in the muscles marked by the prevalent type of oxidation disclosed the higher activity of the cathodic forms--alpha-GDH4 and alpha-GDH5--in the white muscle (m. quadratus lumborum) as compared with the red one (m. soleus). It is suggested that the study of alpha-GDH molecular form distribution may serve as an objective criterion for changes in the ratio of the aerobic and anaerobic processes in animal tissues under different states of animal body.