Tumakov S A, Sidorenkov I V, Pervushkin S V
Biull Eksp Biol Med. 1980 Aug;90(8):170-3.
Electrophoresis and isoelectric focusing in polyacrylamide gel were used to demonstrate the presence of 5 molecular forms of alpha-glycerophosphate dehydrogenase (E. C. I. I. I. 8, alpha-GDH) in the cytoplasm of some vertebrata skeletal muscles. Assay of alpha-GDH isozyme distribution in the muscles marked by the prevalent type of oxidation disclosed the higher activity of the cathodic forms--alpha-GDH4 and alpha-GDH5--in the white muscle (m. quadratus lumborum) as compared with the red one (m. soleus). It is suggested that the study of alpha-GDH molecular form distribution may serve as an objective criterion for changes in the ratio of the aerobic and anaerobic processes in animal tissues under different states of animal body.
采用聚丙烯酰胺凝胶电泳和等电聚焦技术，证实了某些脊椎动物骨骼肌细胞质中存在5种分子形式的α-甘油磷酸脱氢酶（E.C.1.1.1.8，α-GDH）。对以普遍氧化类型为特征的肌肉中α-GDH同工酶分布的测定表明，与红色肌肉（比目鱼肌）相比，白色肌肉（腰方肌）中阴极形式的α-GDH4和α-GDH5活性更高。研究表明，α-GDH分子形式分布的研究可作为动物机体不同状态下动物组织有氧和无氧过程比例变化的客观标准。
