Kimmel D B, Jee W S
Calcif Tissue Int. 1980;32(2):123-33. doi: 10.1007/BF02408531.
The purpose of this work was to provide futher knowledge about bone cell kinetics in the metaphysis of the growing long bone. Seventy rats were sacrificed from 1 to 120 h after injection of tritiated thymidine. Autoradiographs of 3 micrometers thick sections of the proximal tibial metaphysis were studied in a manner which allowed evaluation of labeled cell nuclei as a function of increasing age of metaphyseal tissue. A cell cycle duration study for osteoprogenitor cells was done. Labeled osteoprogenitor cells and osteoblasts first appeared at 1 h post-injection. The great majority of all labeled osteoprogenitor cells and osteoblasts was found within 1 mm of the growth cartilage-metaphyseal junction (GCMJ) at all times, apparently migrating with the moving GCMJ. In contrast, labeled osteoclast nuclei first appeared at 24 h post-injection within 0.3 mm of the GCMJ and remained always with the area of bone surface with which they were first associated, even as the GCMJ migrated away. By 5 days post-injection, the source of new labeled osteoclast nuclei in the metaphysis near the GCMJ was depleted, whereas that for the osteoblasts remained. The existence of two kinetically different, as well as ultrastructurally different, members of the metaphyseal osteoprogenitor cells population is postulated. A cell cycle time of 39 +/- 18 h was found for the osteoprogenitor cell population, but has limited meaning. A schema for metaphyseal bone cell movements during longitudinal bone growth is presented.
这项工作的目的是提供关于生长中长骨干骺端骨细胞动力学的更多知识。在注射氚标记胸腺嘧啶核苷后1至120小时，处死70只大鼠。对胫骨近端干骺端3微米厚切片的放射自显影片进行研究，以便根据干骺端组织年龄的增加来评估标记细胞核。对骨祖细胞进行了细胞周期持续时间研究。标记的骨祖细胞和成骨细胞在注射后1小时首次出现。在所有时间，几乎所有标记的骨祖细胞和成骨细胞都在生长软骨-干骺端交界处（GCMJ）1毫米范围内被发现，显然随着移动的GCMJ迁移。相比之下，标记的破骨细胞核在注射后24小时首次出现在GCMJ 0.3毫米范围内，并始终与它们最初相关的骨表面区域在一起，即使GCMJ迁移离开。注射后5天，GCMJ附近干骺端新的标记破骨细胞核来源耗尽，而成骨细胞的来源仍然存在。推测干骺端骨祖细胞群体存在两种动力学不同以及超微结构不同的成员。发现骨祖细胞群体的细胞周期时间为39±18小时，但意义有限。提出了纵向骨生长过程中干骺端骨细胞运动的模式图。
