Yang Fan, Luo Pengbo, Ding Hao, Zhang Changqing, Zhu Zhenhong
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
Am J Transl Res. 2018 Aug 15;10(8):2469-2479. eCollection 2018.
Collagen is essential for bone adhesion and formation. In the present study, proteomic analysis suggested that collagen type V a2 (COL5A2) was significantly decreased in the necrotic area of patients with steroid-induced necrosis of the femoral head (ONFH). , the effects of methylprednisolone (MP) on the proliferation and differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) were investigated. The expression of the osteogenic-related proteins, Runx2, alkaline phosphatase (ALP), osteocalcin (OC) and COL5A2 was significantly downregulated post-MP treatment. analyses revealed that post-MP treatment, rats showed typical signs of ONFH by micro-CT scanning and hematoxylin and eosin (H&E) staining. Immunohistochemical staining demonstrated that the expression of COL5A2 and vascular endothelial growth factor (VEGF) was significantly decreased post-MP treatment. In conclusion, the expression COL5A2 was lower in patients with steroid-induced ONFH, hence COL5A2 may be a promising therapeutic target for steroid induced ONFH treatment.
胶原蛋白对于骨黏附与形成至关重要。在本研究中，蛋白质组学分析表明，在激素性股骨头坏死（ONFH）患者的坏死区域，Ⅴ型胶原蛋白α2（COL5A2）显著减少。研究了甲基泼尼松龙（MP）对人骨髓间充质干细胞（hBMSCs）增殖与分化的影响。MP处理后，成骨相关蛋白Runx2、碱性磷酸酶（ALP）、骨钙素（OC）和COL5A2的表达显著下调。分析显示，MP处理后，通过显微CT扫描和苏木精-伊红（H&E）染色，大鼠呈现出典型的ONFH体征。免疫组织化学染色表明，MP处理后，COL5A2和血管内皮生长因子（VEGF）的表达显著降低。总之，激素性ONFH患者中COL5A2的表达较低，因此COL5A2可能是激素性ONFH治疗的一个有前景的治疗靶点。
