Ⅴ型胶原α2（COL5A2）在激素性股骨头坏死中表达降低。
Collagen type V a2 (COL5A2) is decreased in steroid-induced necrosis of the femoral head.
作者信息
Yang Fan, Luo Pengbo, Ding Hao, Zhang Changqing, Zhu Zhenhong
机构信息
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.
出版信息
Am J Transl Res. 2018 Aug 15;10(8):2469-2479. eCollection 2018.
Abstract
Collagen is essential for bone adhesion and formation. In the present study, proteomic analysis suggested that collagen type V a2 (COL5A2) was significantly decreased in the necrotic area of patients with steroid-induced necrosis of the femoral head (ONFH). , the effects of methylprednisolone (MP) on the proliferation and differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) were investigated. The expression of the osteogenic-related proteins, Runx2, alkaline phosphatase (ALP), osteocalcin (OC) and COL5A2 was significantly downregulated post-MP treatment. analyses revealed that post-MP treatment, rats showed typical signs of ONFH by micro-CT scanning and hematoxylin and eosin (H&E) staining. Immunohistochemical staining demonstrated that the expression of COL5A2 and vascular endothelial growth factor (VEGF) was significantly decreased post-MP treatment. In conclusion, the expression COL5A2 was lower in patients with steroid-induced ONFH, hence COL5A2 may be a promising therapeutic target for steroid induced ONFH treatment.
摘要
胶原蛋白对于骨黏附与形成至关重要。在本研究中，蛋白质组学分析表明，在激素性股骨头坏死（ONFH）患者的坏死区域，Ⅴ型胶原蛋白α2（COL5A2）显著减少。研究了甲基泼尼松龙（MP）对人骨髓间充质干细胞（hBMSCs）增殖与分化的影响。MP处理后，成骨相关蛋白Runx2、碱性磷酸酶（ALP）、骨钙素（OC）和COL5A2的表达显著下调。分析显示，MP处理后，通过显微CT扫描和苏木精-伊红（H&E）染色，大鼠呈现出典型的ONFH体征。免疫组织化学染色表明，MP处理后，COL5A2和血管内皮生长因子（VEGF）的表达显著降低。总之，激素性ONFH患者中COL5A2的表达较低，因此COL5A2可能是激素性ONFH治疗的一个有前景的治疗靶点。
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