一种源自人葡萄膜黑色素细胞的细胞系的生物学和生物化学特性
Biological and biochemical properties of a human uveal melanocyte-derived cell line.
作者信息
Meyskens F L, Berglund E B, Saxe D F, Fuller B B, Pacelli L Z, Hall J D, Ray C G
出版信息
In Vitro. 1980 Sep;16(9):775-80. doi: 10.1007/BF02619312.
PMID:6773878
Abstract
A human uveal melanocyte-derived cell line (U1I) is described. The cell line has a doubling time of 27.2 hr, a plating efficiency on plastic surfaces of 10%, and a cloning efficiency in soft agar of < 0.1%. U1I displays marked chromosomal aneuploidy and sensitivity to ultraviolet light. Biochemical studies indicate the presence of tyrosinase, which is stimulated by several compounds, including theophylline, progesterone, and nerve growth factor.
摘要
描述了一种源自人葡萄膜黑色素细胞的细胞系（U1I）。该细胞系的倍增时间为27.2小时，在塑料表面的接种效率为10%，在软琼脂中的克隆效率小于0.1%。U1I表现出明显的染色体非整倍性和对紫外线的敏感性。生化研究表明存在酪氨酸酶，它受到几种化合物的刺激，包括茶碱、孕酮和神经生长因子。
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