Neugut A I, Weinstein I B
In Vitro. 1979 May;15(5):351-5. doi: 10.1007/BF02616141.
At the present time, growth in agar suspension is one of the best in vitro correlates of tumorigenicity. Growth in agarose, however, has not been evaluated extensively as an in vitro criterion for tumorigenicity. In the present study we have tested 19 cell lines, including six mouse-human hybrids, for growth in agarose and agar in the presence and absence of exogenous hypoxanthine. None of the six nontumorigenic cell lines grew in agar or agarose. Ten of the 13 tumorigenic cell lines grew in both agar and agarose with about equal efficiency. The remaining three tumorigenic cell lines grew well in agarose but poorly or not at all in agar. Hypoxanthine did not stimulate the growth in agar or agarose of any of the cell lines except BHK. We conclude that growth in agarose may be a more sensitive marker for tumorigenicity than growth in agar and that BHK is exceptional in its sensitivity to supplemental purines.
目前，琼脂悬浮培养中的生长是肿瘤发生性的最佳体外相关指标之一。然而，琼脂糖中的生长尚未作为肿瘤发生性的体外标准进行广泛评估。在本研究中，我们测试了19种细胞系，包括6种小鼠 - 人杂交细胞系，在有和没有外源性次黄嘌呤的情况下在琼脂糖和琼脂中的生长情况。6种非致瘤性细胞系在琼脂或琼脂糖中均未生长。13种致瘤性细胞系中的10种在琼脂和琼脂糖中生长效率大致相同。其余3种致瘤性细胞系在琼脂糖中生长良好，但在琼脂中生长不良或根本不生长。除了BHK细胞系外，次黄嘌呤并未刺激任何细胞系在琼脂或琼脂糖中的生长。我们得出结论，琼脂糖中的生长可能比琼脂中的生长对肿瘤发生性更敏感，并且BHK细胞系对补充嘌呤的敏感性异常。
