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克氏锥虫在条纹臭鼬(Mephitis mephitis)中的实验性感染。

An experimental infection of Trypanosoma cruzi in striped skunks (Mephitis mephitis).

作者信息

Davis D S, Russell L H, Adams L G, Yaeger R G, Robinson R M

出版信息

J Wildl Dis. 1980 Jul;16(3):403-6. doi: 10.7589/0090-3558-16.3.403.

DOI:10.7589/0090-3558-16.3.403
PMID:6774112
Abstract

Four adult (3 male, 1 female) captive-raised, decented, striped skunks (Mephritis mephitis) were infected experimentaly with a field strain (Texas-Tulane) of Trypanosoma cruzi, originally isolated from a naturally-infected dog. Two skunks were injected intravenously with approximately 4.5 x 10(6) viable T. cruzi trypomastigotes. Two skunks were inoculated per os and per conjunctivum with 10 ml of phosphate buffered saline containing macerated, T. cruzi-infected triatomine intestines and intestinal contents. The skunks had minimal clinical manifestations with no mortalities occurring during 46 days post-exposure. Sera from all skunks were positive at 24 days post-inoculation (PI) by the direct and latex agglutination tests. Blood cultures from the 4 skunksd were positive for T. cruzi at day 24 PI and 3 were positive at day 46 PI. All skunks had mild to moderately severe chronic granulomatous myocarditis of the atria and ventricles. Typical T. cruzi amastigotes were present within myocardial fibers in 3 of 4 skunks.

摘要

四只成年(3只雄性,1只雌性)人工饲养、健康的条纹臭鼬(Mephritis mephitis)被实验性感染克氏锥虫的一个野外毒株(德克萨斯-杜兰株),该毒株最初从一只自然感染的狗身上分离得到。两只臭鼬通过静脉注射约4.5×10⁶个活的克氏锥虫锥鞭毛体。另外两只臭鼬经口和结膜接种10毫升含有研磨后的、感染克氏锥虫的锥蝽肠道及肠道内容物的磷酸盐缓冲盐水。这些臭鼬临床表现轻微,在暴露后46天内无死亡发生。所有臭鼬的血清在接种后24天通过直接凝集试验和乳胶凝集试验呈阳性。4只臭鼬的血培养在接种后24天克氏锥虫呈阳性,3只在接种后46天呈阳性。所有臭鼬均有轻至中度严重的心房和心室慢性肉芽肿性心肌炎。4只臭鼬中有3只的心肌纤维内存在典型的克氏锥虫无鞭毛体。

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