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紫外线辐射对表面进行消毒和灭菌的研究(作者译)

[Investigations on disinfection and sterilization of surfaces by ultraviolet radiation (author's transl)].

作者信息

Dietz P, Böhm R, Strauch D

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B. 1980;171(2-3):158-67.

PMID:6774514
Abstract

Some model investigations have been made in order to find out if disinfection or sterilization in an ultraviolet radiation cabinet (fig. 1) is effective. The reduction rate of spores of Bacillus anthracis dried to germ carriers of aluminium, ceramics or wood was used for indicating the germicidal effect. It was found, that a 7.5 times higher dose of radiation was necessary to reach a similar reduction rate of spores on germ carriers of ceramics as it was found for the ones of aluminium (tab. 1 and 2). Very high dosage of radiation was necessary to get a safe reduction of spores on each germ carrier (fig. 2 and 3). On wooden germ carriers an average logarithmical reduction (R) of no more than 0.67 was found after 30 h of exposure (tab. 3). Unreliable results have been found in the sterilization experiments with spore bearing germ carriers of aluminium and ceramics. In a few rare cases sterility could be obtained by an ultraviolet radiation dose of 2.000.000 microW s/cm2 (tab. 4). But generally a dosage of even 222.600.000 microW s/cm2 was not sufficient to sterilize the copntaminated germ carriers. The ultraviolet radiation cabinet cannot be recommended for disinfection or sterilization.

摘要

为了确定紫外线辐射箱(图1)中的消毒或灭菌是否有效,已进行了一些模型研究。炭疽芽孢杆菌的孢子干燥到铝、陶瓷或木质的菌载体上后的减少率被用来指示杀菌效果。结果发现,要使陶瓷菌载体上的孢子减少率达到与铝菌载体上相似的水平,所需的辐射剂量要高7.5倍(表1和表2)。要在每种菌载体上安全减少孢子数量,需要非常高的辐射剂量(图2和图3)。在木质菌载体上,暴露30小时后平均对数减少量(R)不超过0.67(表3)。在对带有铝和陶瓷孢子的菌载体进行的灭菌实验中,得到的结果不可靠。在极少数情况下,通过2000000微瓦·秒/平方厘米的紫外线辐射剂量可实现无菌状态(表4)。但一般来说,即使是222600000微瓦·秒/平方厘米的剂量也不足以对受污染的菌载体进行灭菌。紫外线辐射箱不推荐用于消毒或灭菌。

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