Filippov Iu V, Efimov D D, Belova T N, Zvenigorodskaia V P
Zh Mikrobiol Epidemiol Immunobiol. 1980 May(5):77-82.
The peptide composition and the molecular weight of peptides were determined in meningococci of serogroup A (7 strains), serogroup B (7 strains; of these, 4 strains belonged to serotype 2), serogroup C (4 strains) and serogroups D, X. Y and Z (1 strain each) by means of electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. All serogroup A strains were found to contain 2 main peptides with molecular weights of 39,000 and 45,000 daltons; molecular weights of peptides in other serogroups varied in different strains. In group B and C meningococci, besides the reference strains of serotype 2, 4 more strains with peptide characteristics allowing to consider these strains belonging to serotype 2 were detected. The serotype-specific high-molecular protein preparation, purified to a considerable extent from lipopolysaccharide and hydrocarbons, was obtained from serogroup B strain, serotype 2. This preparation, when used for the immunization of rabbits, induced the formation of serotype-specific antibodies. The high-molecular protein preparation, serotype 2, was found to contain 5 individual antigenic components.
通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳法，对A群（7株）、B群（7株；其中4株属于2型）、C群（4株）以及D群、X群、Y群和Z群（各1株）脑膜炎球菌的肽组成和肽分子量进行了测定。发现所有A群菌株均含有2种主要肽，分子量分别为39,000和45,000道尔顿；其他血清群中肽的分子量在不同菌株中有所不同。在B群和C群脑膜炎球菌中，除了2型参考菌株外，还检测到另外4株具有肽特征的菌株，可认为这些菌株属于2型。从B群2型菌株中获得了在很大程度上从脂多糖和碳氢化合物中纯化出来的血清型特异性高分子蛋白质制剂。该制剂用于免疫兔子时，可诱导血清型特异性抗体的形成。发现2型高分子蛋白质制剂含有5种单独的抗原成分。
