Riccardi V M, Maragos V A
In Vitro. 1980 Aug;16(8):706-14. doi: 10.1007/BF02619200.
The in vitro expression of the autosomal dominant mutation responsible for neurofibromatosis was probed using the amino acid analogue 3-nitrotyrosine as a cell culture selective agent. The presence of 3-nitrotyrosine in culture medium led to inhibition of growth and cell death among normal skin fibroblasts in log phase growth, whereas cell strains derived from six different patients' neurofibromas or skin cells, or both, exhibited a consistently enhanced ability to survive under the same conditions. At 0.8 mM 3-nitrotyrosine, four patient-derived skin fibroblast strains could be differentiated from five strains of control skin fibroblasts with a high level of confidence (P < 0.0000). In the same way four neurofibroma-derived fibroblast strains were differentiated from control skin fibroblasts (P < 0.0022). Neurofibroma-derived cells were not different from control cells when treated with 5-fluorotryptophan or p-fluorophenylalanine.
使用氨基酸类似物3-硝基酪氨酸作为细胞培养选择剂，对导致神经纤维瘤病的常染色体显性突变的体外表达进行了探究。培养基中3-硝基酪氨酸的存在导致对数期生长的正常皮肤成纤维细胞生长受到抑制并发生细胞死亡，而源自6名不同患者神经纤维瘤或皮肤细胞或两者的细胞株在相同条件下表现出持续增强的存活能力。在3-硝基酪氨酸浓度为0.8 mM时，4株源自患者的皮肤成纤维细胞株能够与5株对照皮肤成纤维细胞株高度可靠地区分开来（P < 0.0000）。同样，4株源自神经纤维瘤的成纤维细胞株也能与对照皮肤成纤维细胞区分开来（P < 0.0022）。当用5-氟色氨酸或对氟苯丙氨酸处理时，源自神经纤维瘤的细胞与对照细胞没有差异。
