小牛胸腺细胞质膜中糖蛋白的天冬酰胺连接糖链。中性寡糖的结构研究。

The asparagine-linked sugar chains of the glycoproteins in calf thymocyte plasma membrane. Structural studies of neutral oligosaccharides.

作者信息

Yoshima H, Takasaki S, Kobata A

出版信息

J Biochem. 1980 Sep;88(3):819-27. doi: 10.1093/oxfordjournals.jbchem.a133036.

DOI:10.1093/oxfordjournals.jbchem.a133036

Abstract

The neutral oligosaccharide fraction obtained from the hydrazinolysate of the plasma membrane glycoproteins of calf thymocytes was shown to be a mixture of twelve oligosaccharides. Oligosaccharides A, B, and C, which released galactose on treatment with jack bean beta-galactosidase but not with diplococcal beta-galactosidase, were shown to have the structures Gal beta 1-3Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-6(3)[Gal beta 1-3Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-3(6)]Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, Gal beta 1-3Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-6(3)[Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3(6)]Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, and Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-3Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc, respectively. Oligo saccharides D and E, which released galactose on treatment with either jack bean beta-galactosidase or diplococcal beta-galactosidase, were shown to have the structures Gal beta 1-4GlcNAc beta 1-2Man alpha 1-(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc and Gal beta 1-4GlcNAc beta 1-2Man alpha 1-(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc, respectively. Five oligosaccharides (F1, G, H1, I, and J) were shown to have typical high-mannose type structures (Man alpha 1-)n.Man beta 1-4GlcNAc beta 1-GlcNAc, with n-values of 8, 7, 6, 5, and 4, respectively. The structures of the remaining two oligosaccharides F2 and H2 were elucidated as GlcNAc beta 1-2Man alpha 1-(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-)GlcNAc and Man alpha 1-(Man alpha 1-)Man beta 1-(GlcNAc beta 1-Man alpha 1-)Man beta 1-GlcNAc beta 1-GlcNAc, respectively.

摘要

从小牛胸腺细胞质膜糖蛋白的肼解产物中获得的中性寡糖部分被证明是12种寡糖的混合物。寡糖A、B和C在用刀豆β-半乳糖苷酶处理时会释放半乳糖，但用双球菌β-半乳糖苷酶处理时则不会，它们的结构分别为Galβ1-3Galβ1-4GlcNAcβ1-4（Galβ1-3Galβ1-4GlcNAcβ1-2）Manα1-6(3)[Galβ1-3Galβ1-4GlcNAcβ1-6（Galβ1-3Galβ1-4GlcNAcβ1-2）Manα1-3(6)]Manβ1-4GlcNAcβ1-4（Fucα1-6）GlcNAc、Galβ1-3Galβ1-4GlcNAcβ1-4（Galβ1-3Galβ1-4GlcNAcβ1-2）Manα1-6(3)[Galβ1-3Galβ1-4GlcNAcβ1-2Manα1-3(6)]Manβ1-4GlcNAcβ1-4（Fucα1-6）GlcNAc和Galβ1-3Galβ1-4GlcNAcβ1-2Manα1-6（Galβ1-3Galβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4（Fucα1-6）GlcNAc。寡糖D和E在用刀豆β-半乳糖苷酶或双球菌β-半乳糖苷酶处理时都会释放半乳糖，它们的结构分别为Galβ1-4GlcNAcβ1-2Manαi-（Galβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4（Fucα1-）GlcNAc和Galβ1-4GlcNAcβ1-2Manα1-（GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4（Fucα1-）GlcNAc。5种寡糖（F1、G、H1、I和J）被证明具有典型的高甘露糖型结构（Manα1-）n.Manβ1-4GlcNAcβ1-GlcNAc,n值分别为8、7、6、5和4。其余两种寡糖F2和H2的结构分别被阐明为GlcNAcβ1-2Manα1-（GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4（Fucα1-）GlcNAc和Manα1-（Manα1-）Manβ1-（GlcNAcβ1-Manα1-）Manβ1-GlcNAcβ1-GlcNAc。