一种从单核细胞增生李斯特菌中分离噬菌体的简单方法。
A simple method for the isolation of phages from Listeria monocytogenes.
作者信息
Durst J, Rau E, Kemenes F, Berencsi G
出版信息
Zentralbl Bakteriol A. 1980 Feb;246(1):23-5.
PMID:6775437
Abstract
By application of a combined mitomycin-/heat treatment after freezing, 7 out of 29 Listeria monocytogenes strains which were found to be non-phage carriers by UV irradiation could release phages. Propagation of the obtained phages was promoted by storage at 4 degrees C. Apart from TNSA plates the application of chocolate plates appears to be necessary in order to study these phages.
摘要
冷冻后应用丝裂霉素和热处理相结合的方法，29株经紫外线照射被发现为非噬菌体携带者的单核细胞增生李斯特菌中有7株能够释放噬菌体。将获得的噬菌体在4℃保存可促进其增殖。为了研究这些噬菌体，除了TNSA平板外，似乎还需要使用巧克力平板。
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