Gerner-Smidt P, Rosdahl V T, Frederiksen W
Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
APMIS. 1993 Feb;101(2):160-7.
A phage typing system was developed for Listeria monocytogenes. Phages were released from clinical and from food strains of L. monocytogenes by mitomycin C induction. The system consists of two subsystems, one of which is used to type L. monocytogenes serotype 1 strains, containing 12 phages, and a second which is used to type serotype 4 strains, containing 14 other phages. For the serotype 1 subsystem, the reproducibility was > or = 90%, the typability 92%, and the discriminatory power, as judged by the discriminatory index, 80%. The corresponding figures for the serotype 4 subsystem were: reproducibility > or = 94%, typability 87%, and discriminatory index 87%. The performance of the whole typing system is sufficient for it to be used for screening purposes.
开发了一种用于单核细胞增生李斯特菌的噬菌体分型系统。通过丝裂霉素C诱导从单核细胞增生李斯特菌的临床菌株和食品菌株中释放噬菌体。该系统由两个子系统组成，其中一个用于对1型单核细胞增生李斯特菌菌株进行分型，包含12种噬菌体，另一个用于对4型菌株进行分型，包含另外14种噬菌体。对于1型子系统，重复性≥90%，分型能力为92%，通过鉴别指数判断的鉴别力为80%。4型子系统的相应数据为：重复性≥94%，分型能力87%，鉴别指数87%。整个分型系统的性能足以用于筛查目的。
