Vargas F F, Blackshear G L, Majerle R J
Am J Physiol. 1980 Oct;239(4):H464-H468. doi: 10.1152/ajpheart.1980.239.4.H464.
Rabbits were anesthetized with Nembutal; their hearts were removed and perfused with Ringers solution. Osmotic weight transients were then produced by test solute infusion into the perfusate. The rate constant for organ weight change was used to predict test solute venous concentration (CV) and the distribution volume (V) during an osmotic transient by use of the Johnson and Wilson (6) model for capillary tissue exchange. By use of Cr EDTA, strongly purple in solution, we compared the above predictions with a value of CV obtained directly by optical measurement of outflow venous concentration and values of V calculated from our measurements of organ weight and the known sucrose distribution volume. The close agreement between both sets of values with a permeability coefficient of 5 x 10(-5) cm/s and a volume of distribution of 3.2 ml/10 g heart lead us to conclude that the model used closly represents the conditions of the isolated perfused heart, and that both the osmotic transient and the extraction measurements provide good estimates of organ capillary permeability.
兔子用戊巴比妥麻醉；取出它们的心脏并用林格氏液灌注。然后通过向灌注液中注入测试溶质产生渗透重量瞬变。通过使用约翰逊和威尔逊（6）的毛细血管组织交换模型，利用器官重量变化的速率常数来预测渗透瞬变期间测试溶质的静脉浓度（CV）和分布容积（V）。通过使用溶液中呈强紫色的铬乙二胺四乙酸，我们将上述预测值与通过光学测量流出静脉浓度直接获得的CV值以及根据我们对器官重量的测量和已知蔗糖分布容积计算出的V值进行了比较。两组值之间的密切一致性，渗透系数为5×10（-5）cm/s，分布容积为3.2 ml/10 g心脏，使我们得出结论，所使用的模型紧密代表了离体灌注心脏的情况，并且渗透瞬变和提取测量都能很好地估计器官毛细血管通透性。
