Picorel R, Del Campo F F, Ramirez J M, Gingras G
Biochim Biophys Acta. 1980 Nov 5;593(1):76-84. doi: 10.1016/0005-2728(80)90009-2.
Rhodospirillum rubrum strain F24.1 is a spontaneous revertant of nonphototrophic mutant F24 derived from wild-type strain S1. Strain F24 shows no detectable photochemical activity and contains, at most, traces of the photoreaction center polypeptides. Strain F24.1 has a phototrophic growth rate close to that of the wild-type strain (Picorel, R., del Valle-Tascón, S. and Ramírez, J.M. (1977) Arch. Biophys. Biochem. 181, 665-670) but shows little photochemical activity. Light-induced absorbance changes in the near-infrared, photoinduced EPR signals and ferricyanide-elicited absorbance changes indicate that strain F24.1 has a photoreaction center content of 7-8% as compared to strain S1. Polyacrylamide gel electrophoresis of isolated F24.1 chromatophores shows the photoreaction center polypeptides to be present in amounts compatible with this value. Photoreaction center was prepared from strain F24.1 and showed no detectable difference with that of strain S1. It is concluded that strain F24.1 photosynthesis is due entirely to its residual 7-8% of typical photoreaction center.
深红螺菌F24.1菌株是源自野生型菌株S1的非光合突变体F24的自发回复突变体。F24菌株未检测到光化学活性，最多仅含有痕量的光反应中心多肽。F24.1菌株的光合生长速率接近野生型菌株（皮科雷尔，R.，德尔巴列 - 塔斯科恩，S.和拉米雷斯，J.M.（1977年）《生物物理与生物化学文献》181，665 - 670），但光化学活性较低。近红外光诱导的吸光度变化、光诱导的电子顺磁共振信号以及铁氰化物引发的吸光度变化表明，与S1菌株相比，F24.1菌株的光反应中心含量为7 - 8%。对分离出的F24.1菌绿体进行聚丙烯酰胺凝胶电泳显示，光反应中心多肽的含量与此值相符。从F24.1菌株制备的光反应中心与S1菌株的光反应中心未检测到差异。得出的结论是，F24.1菌株的光合作用完全归因于其残留的7 - 8%的典型光反应中心。
