Vadeboncoeur C, Noël H, Poirier L, Cloutier Y, Gingras G
Biochemistry. 1979 Oct 2;18(20):4301-8. doi: 10.1021/bi00587a007.
The photoreaction center from Rhodospirillum rubrum contains about 90% protein, 6% pigment, mere traces of lipids, and no cytochromes. It also contains at least 1 mol of ubiquinone and 1 iron atom per mol. Its three-component polypeptide chains were isolated by preparative electrophoresis, and their molar stoichiometry was established as 1:1:1. The amino acid composition of the photoreaction center from strain S1 and from its subunits is reported. The protein as a whole contains about 65% nonpolar residues, and the degree of hydrophobicity of its subunits is alpha less than beta less than gamma. The minimal molecular weight based on the extinction coefficient and on the amino acid content is 90 000. This corresponds to a half-cystine mole number of 6.
红螺菌的光反应中心含有约90%的蛋白质、6%的色素、微量的脂质，且不含细胞色素。每摩尔还含有至少1摩尔的泛醌和1个铁原子。其由三条多肽链组成的成分通过制备电泳分离出来，其摩尔化学计量比确定为1:1:1。报道了S1菌株光反应中心及其亚基的氨基酸组成。整个蛋白质含有约65%的非极性残基，其亚基的疏水性程度为α＜β＜γ。基于消光系数和氨基酸含量得出的最小分子量为90000。这对应于6个半胱氨酸摩尔数。
