Raisys V A, Friel P N, Graaff P R, Opheim K E, Wilensky A J
J Chromatogr. 1980 Oct 10;183(4):441-8. doi: 10.1016/s0378-4347(00)81586-8.
A one-step method for extraction of diazepam, nordiazepam, and internal standard into toluene is followed by chromatographic separation and detection with either dual-wave-length high-performance liquid chromatography or electron-capture gas-liquid chromatography. Agreement between the two methods was excellent for diazepam (r = 0.99, n = 38) and good for nordiazepam (r = 0.96, n = 79) over a concentration range that included subtherapeutic, therapeutic, and toxic plasma levels.
