血清中地西泮及其主要代谢物的液相色谱测定法，及其在精神分裂症患者高剂量地西泮药代动力学研究中的应用。
Liquid-chromatographic assay of diazepam and its major metabolites in serum, and application to pharmacokinetic study of high doses of diazepam in schizophrenics.
作者信息
Tada K, Moroji T, Sekiguchi R, Motomura H, Noguchi T
出版信息
Clin Chem. 1985 Oct;31(10):1712-5.
In this rapid, sensitive method for simultaneously determining diazepam and its biologically active metabolites--nordiazepam, oxazepam, and temazepam--in serum, 250 to 1000 microL of serum is extracted with ether and the extracted compounds are quantified by "high-performance" liquid chromatography on a Shimpack FLC-C8 microparticulate column. Absorbance of the effluent is monitored at 254 nm. The limit of detection in serum is about 10 micrograms/L for each drug. Analytical recovery of each drug added to the serum varied from 91 to 102% for oxazepam and temazepam, from 82 to 99% for nordiazepam and diazepam. Within-day and between-day CVs ranged from 2 to 5% for oxazepam and temazepam, and from 2 to 7% for nordiazepam and diazepam. We also report results on using this assay in a pharmacokinetic study of high doses of diazepam used to treat chronic schizophrenic patients.
在这种用于同时测定血清中地西泮及其生物活性代谢物——去甲地西泮、奥沙西泮和替马西泮的快速、灵敏方法中，取250至1000微升血清用乙醚萃取，萃取后的化合物通过在Shimpack FLC-C8微粒柱上的“高效”液相色谱法定量。在254纳米处监测流出物的吸光度。血清中每种药物的检测限约为10微克/升。添加到血清中的每种药物的分析回收率，奥沙西泮和替马西泮为91%至102%，去甲地西泮和地西泮为82%至99%。奥沙西泮和替马西泮日内和日间变异系数范围为2%至5%，去甲地西泮和地西泮为2%至7%。我们还报告了在一项用于治疗慢性精神分裂症患者的高剂量地西泮药代动力学研究中使用该检测方法的结果。
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