嗜甲基甲基ophilus菌对甲醇转化为单细胞蛋白的转化率提高。
Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.
作者信息
Windass J D, Worsey M J, Pioli E M, Pioli D, Barth P T, Atherton K T, Dart E C, Byrom D, Powell K, Senior P J
出版信息
Nature. 1980 Oct 2;287(5781):396-401. doi: 10.1038/287396a0.
DOI:10.1038/287396a0
PMID:6776410
Abstract
The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus. Assimilation of ammonia via glutamate dehydrogenase is more energy-efficient than via glutamate synthase, thus the recombinant organism converts more growth substrate, methanol, into cellular carbon.
摘要
大肠杆菌的谷氨酸脱氢酶基因已被克隆到广泛宿主范围的质粒中，并能互补嗜甲基甲基ophilus的谷氨酸合酶突变体。通过谷氨酸脱氢酶同化氨比通过谷氨酸合酶更节能，因此重组生物体将更多的生长底物甲醇转化为细胞碳。
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