将大肠杆菌gdhA基因导入菜豆根瘤菌：对固氮作用的影响
Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation.
作者信息
Bravo A, Becerril B, Mora J
机构信息
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
出版信息
J Bacteriol. 1988 Feb;170(2):985-8. doi: 10.1128/jb.170.2.985-988.1988.
Abstract
Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis. Nodules with bacteroids that expressed GDH activity had severe impairment of nitrogen fixation. Also, R. phaseoli cells that lost GDH activity and assimilated ammonium by the glutamine synthetase-glutamate synthase pathway preferentially nodulated Phaseolus vulgaris.
摘要
菜豆根瘤菌缺乏谷氨酸脱氢酶（GDH），通过谷氨酰胺合成酶-谷氨酸合酶途径同化铵。构建了一株携带大肠杆菌GDH结构基因（gdhA）的菜豆根瘤菌菌株。GDH活性在自由生活状态和共生状态的菜豆根瘤菌中均有表达。具有表达GDH活性类菌体的根瘤固氮严重受损。此外，失去GDH活性并通过谷氨酰胺合成酶-谷氨酸合酶途径同化铵的菜豆根瘤菌细胞优先与菜豆形成根瘤。
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