硫酸软骨素蛋白聚糖核心蛋白的单克隆抗体。
Clonal antibodies for core protein of chondroitin sulfate proteoglycan.
作者信息
Dorfman A, Hall T, Ho P L, Fitch F
出版信息
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3971-3. doi: 10.1073/pnas.77.7.3971.
Abstract
Antibodies have been prepared to the core protein of cartilage chondroitin sulfate proteoglycan from clones isolated after fusion of rat spleen cells and mouse myeloma cell lines. Antibodies produced by five clones were studied in detail by a radioimmunoassay utilizing 35SO42-labeled hyaluronidase-digested chondroitin sulfate proteoglycan. Preparations of antigen were shown to contain at least two antigenic determinants, one of which was restricted to a portion of antigen in these preparations. One clone was shown to react with proteoglycan synthesized in a cell-free system. It is proposed that such clonal antibodies can be used for structural and biosynthetic studies of core protein.
摘要
利用大鼠脾细胞与小鼠骨髓瘤细胞系融合后分离得到的克隆，已制备出针对软骨硫酸软骨素蛋白聚糖核心蛋白的抗体。通过使用35SO42标记的透明质酸酶消化的硫酸软骨素蛋白聚糖的放射免疫测定法，对五个克隆产生的抗体进行了详细研究。抗原制剂显示含有至少两种抗原决定簇，其中一种仅限于这些制剂中的部分抗原。已证明一个克隆与无细胞系统中合成的蛋白聚糖发生反应。有人提出，这种克隆抗体可用于核心蛋白的结构和生物合成研究。
相似文献
1
Clonal antibodies for core protein of chondroitin sulfate proteoglycan.硫酸软骨素蛋白聚糖核心蛋白的单克隆抗体。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3971-3. doi: 10.1073/pnas.77.7.3971.
2
Immunofluorescence studies of chondroitin sulfate proteoglycan biosynthesis: the use of monoclonal antibodies.硫酸软骨素蛋白聚糖生物合成的免疫荧光研究：单克隆抗体的应用。
Coll Relat Res. 1984 Jan;4(1):1-20. doi: 10.1016/s0174-173x(84)80025-4.
3
Chondroitin 6-sulfate oligosaccharides as immunological determinants of chick proteoglycans.硫酸软骨素6-硫酸寡糖作为鸡蛋白聚糖的免疫决定簇
J Biol Chem. 1981 Aug 25;256(16):8279-82.
4
5
Immunological evidence for two distinct chondroitin sulfate proteoglycan core proteins: differential expression in cartilage matrix deficient mice.两种不同硫酸软骨素蛋白聚糖核心蛋白的免疫学证据：在软骨基质缺陷小鼠中的差异表达
Dev Biol. 1983 Jul;98(1):139-47. doi: 10.1016/0012-1606(83)90342-1.
6
Presence of the HNK-1 epitope on poly(N-acetyllactosaminyl) oligosaccharides and identification of multiple core proteins in the chondroitin sulfate proteoglycans of brain.
Biochemistry. 1989 May 16;28(10):4468-74. doi: 10.1021/bi00436a052.
7
Immunocytochemical investigation on the distribution of small chondroitin sulfate-dermatan sulfate proteoglycan in the human.人中小硫酸软骨素-硫酸皮肤素蛋白聚糖分布的免疫细胞化学研究
J Histochem Cytochem. 1986 Aug;34(8):1013-9. doi: 10.1177/34.8.2426331.
8
9
Proteoglycan biosynthesis in chondrocytes: protein A-gold localization of proteoglycan protein core and chondroitin sulfate within Golgi subcompartments.软骨细胞中的蛋白聚糖生物合成：高尔基亚区室中蛋白聚糖蛋白核心和硫酸软骨素的蛋白A-金定位
J Cell Biol. 1985 Dec;101(6):2355-65. doi: 10.1083/jcb.101.6.2355.
10
Antibodies to three chondroitin sulfate-containing proteoglycans in squid skin recognize hexa- or longer chondroitin oligosaccharides as major antigenic determinants.针对鱿鱼皮肤中三种含硫酸软骨素蛋白聚糖的抗体将六糖或更长的硫酸软骨素寡糖识别为主要抗原决定簇。
Arch Biochem Biophys. 1995 Jan 10;316(1):100-9. doi: 10.1006/abbi.1995.1015.
引用本文的文献
1
Aggrecan: A Target Molecule of Autoimmune Reactions.聚集蛋白聚糖：自身免疫反应的靶分子。
Pathol Oncol Res. 1996;2(4):219-228. doi: 10.1007/BF02904814.
2
Cell-free translation of messenger RNA for chondroitin sulphate proteoglycan core protein in rat cartilage.大鼠软骨中硫酸软骨素蛋白聚糖核心蛋白信使核糖核酸的无细胞翻译
Biochem J. 1984 Jan 1;217(1):259-63. doi: 10.1042/bj2170259.
3
Localization of antigenic components in proteoglycan aggregate of bovine nasal cartilage.牛鼻软骨蛋白聚糖聚集体中抗原成分的定位
Histochemistry. 1983;77(2):217-32. doi: 10.1007/BF00506565.
本文引用的文献
1
A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
2
Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic acid interaction.软骨蛋白聚糖的聚集。II. 蛋白聚糖-透明质酸相互作用的寡糖竞争者。
J Biol Chem. 1974 Jul 10;249(13):4242-9.
3
Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.大鼠免疫球蛋白的三个类别和四个（亚）类别：IgM、IgA、IgE以及IgG1、IgG2a、IgG2b、IgG2c。
Eur J Immunol. 1974 Jan;4(1):44-8. doi: 10.1002/eji.1830040112.
4
The use of protein A-containing Staphylococcus aureus as a solid phase anti-IgG reagent in radioimmunoassays as exemplified in the quantitation of alpha-fetoprotein in normal human adult serum.含蛋白A的金黄色葡萄球菌作为放射免疫测定中的固相抗IgG试剂的应用，如在正常成人血清中甲胎蛋白定量测定中所示。
Eur J Immunol. 1974 Jan;4(1):29-33. doi: 10.1002/eji.1830040108.
5
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
6
Classes and subclasses of rat immunoglobulins: interaction with the complement system and with staphylococcal protein A.大鼠免疫球蛋白的类别和亚类：与补体系统及葡萄球菌蛋白A的相互作用
Immunochemistry. 1978 Feb;15(2):125-9. doi: 10.1016/0161-5890(78)90052-4.
7
Properties of rat anti-MHC antibodies produced by cloned rat-mouse hybridomas.克隆化大鼠-小鼠杂交瘤产生的大鼠抗MHC抗体的特性
Immunol Rev. 1979;47:91-115. doi: 10.1111/j.1600-065x.1979.tb00290.x.
8
Simultaneous localization of type II collagen and core protein of chondroitin sulfate proteoglycan in individual chondrocytes.硫酸软骨素蛋白聚糖核心蛋白与Ⅱ型胶原蛋白在单个软骨细胞中的同时定位
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1261-4. doi: 10.1073/pnas.76.3.1261.
9
Translation and characterization of messenger RNAs in differentiating chicken cartilage.分化中的鸡软骨中信使核糖核酸的翻译与特性分析
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4847-51. doi: 10.1073/pnas.76.10.4847.
10
Effect of reduction and alkylation on the antigenicity of cartilage proteoglycans.还原和烷基化对软骨蛋白聚糖抗原性的影响。
Biochem Biophys Res Commun. 1979 Aug 28;89(4):1193-9. doi: 10.1016/0006-291x(79)92134-x.
Zotero 插件