Vertel B M, Upholt W B, Dorfman A
Biochem J. 1984 Jan 1;217(1):259-63. doi: 10.1042/bj2170259.
Total RNA was extracted from the cartilage tissues rat Swarm chondrosarcoma, neonatal-rat breastplate and embryonic-chicken sterna and translated in wheat-germ cell-free reactions. The core protein of the chondroitin sulphate proteoglycan subunit was identified among translation products of rat mRNA by its apparent Mr of 330 000 and by its immunoprecipitation with specific antisera prepared against rat or chicken proteoglycan antigens. The apparent Mr of the rat proteoglycan core protein is 8000-10000 less than that of the equivalent chicken cartilage core-protein product.
从大鼠Swarm软骨肉瘤、新生大鼠胸骨和胚胎鸡胸骨的软骨组织中提取总RNA，并在麦胚无细胞反应体系中进行翻译。硫酸软骨素蛋白聚糖亚基的核心蛋白在大鼠mRNA的翻译产物中得以鉴定，其表观分子量为330000，并且可被针对大鼠或鸡蛋白聚糖抗原制备的特异性抗血清免疫沉淀。大鼠蛋白聚糖核心蛋白的表观分子量比相应的鸡软骨核心蛋白产物小8000 - 10000。
