Kurnosova L M, Sukhodol'skaia A E, Suvorova T I, Faustova V A
Zh Mikrobiol Epidemiol Immunobiol. 1980 Jul(7):8-13.
Using the method proposed by Gillies and Govan and their indicator strains, 342 P. aeruginosa strains isolated from the patients were studied in respect to their pyocinogenicity and typed according to the production of different types of pyocins. Besides, in 206 cultures the pyocin sensitivity of 16 standard P. aeruginosa strains (5 strains obtained from Govan and 11 strains provided by the authors) was determined. All the tested cultures fell into 23 pyocin types; of these, types I and X occured most frequently, 56 strains identified by means of indicators could not be typed due to the fact that the corresponding pyocin types were absent in Govan's scheme. The cultures isolated from the patients and the environmental objects during the outbreak of P. aeruginosa in a hospital were proved to belong to the same pyocin type (III). The double typing of the cultures, according to pyocin production and pyocin sensitivity, allowed to determine individual characteristics of 75% of the tested cultures.
采用吉利斯和戈万提出的方法及其指示菌株,对从患者中分离出的342株铜绿假单胞菌进行了产绿脓菌素能力的研究,并根据不同类型绿脓菌素的产生情况进行分型。此外,还测定了206份培养物中16株标准铜绿假单胞菌菌株(5株来自戈万,11株由作者提供)对绿脓菌素的敏感性。所有测试培养物分为23种绿脓菌素类型;其中,I型和X型出现频率最高,56株通过指示菌株鉴定的菌株无法分型,原因是戈万的分类方案中没有相应的绿脓菌素类型。在一家医院铜绿假单胞菌暴发期间从患者和环境物体中分离出的培养物被证明属于同一绿脓菌素类型(III型)。根据绿脓菌素产生情况和绿脓菌素敏感性对培养物进行双重分型,能够确定75%测试培养物的个体特征。