Kantarci G
Ege Universitesi, Eczacilik Fakültesi Farmasötik Teknoloji Anabilim Dali Araştirma Görevlisi, Mikrobiyoloji Doktoru.
Mikrobiyol Bul. 1992 Jan;26(1):17-25.
In our study, 103 strains of P.aeruginosa from various clinical specimens were typed by pyocin typing method with 13 indicator strains. As a result of pyocin typing, 18 different pyocin type were detected from 81 typable strains (78.6%), 22 of P.aeruginosa strains (21.3%) could not be typed because of the lack of their pyocin activity. On the other hand, 2 of typable P.aeruginosa strains were found to produce inhibition patterns that differed from standard inhibition patterns according to Govan and Gillies Method. Because of this reason, these two strains could not be classified. As a result of our study, it was observed that, strains that belong to pyocin type-1 were in majority. It was detected that, TSA Medium supplemented with 5% sheep blood can be used easily instead of TSA Medium, supplemented with 5% horse blood.
在我们的研究中,使用13株指示菌株,通过绿脓菌素分型法对来自各种临床标本的103株铜绿假单胞菌进行了分型。绿脓菌素分型的结果显示,在81株可分型菌株(78.6%)中检测到18种不同的绿脓菌素类型,22株铜绿假单胞菌菌株(21.3%)由于缺乏绿脓菌素活性而无法分型。另一方面,发现2株可分型的铜绿假单胞菌菌株产生的抑制模式与根据戈万和吉利斯方法的标准抑制模式不同。由于这个原因,这两株菌株无法分类。我们的研究结果表明,绿脓菌素1型的菌株占多数。还检测到,添加5%羊血的胰酪大豆胨培养基可以很容易地替代添加5%马血的胰酪大豆胨培养基。