Kovács P, Csaba G
Acta Histochem. 1980;66(1):50-8. doi: 10.1016/S0065-1281(80)80082-1.
An anti-histaminic immune serum, prepared in New Zealand male rabbits by immunization with a chemospecific antigen (diazotized para-amino-benzoyl-azohistamine) was labeled with fluorescein isothiocyanate for the purpose of histamine detection or was employed for demonstration of histamine using fluorescein-labeled protein A. The IgG fraction of the hyperimmune serum was isolated, purified, and tested for specificity by binding to 3H-histamine or to histamine-containing or histamine-treated cells. Histological preparations used for detection of histamine should be fixed preferably in Carnoy solution. The procedure can also be employed for ultra-histochemical detection of histamine, using ferritin-conjugated antibody or the peroxidase technique.
通过用化学特异性抗原（重氮化对氨基苯甲酰偶氮组胺）免疫新西兰雄性兔制备的抗组胺免疫血清，为检测组胺而用异硫氰酸荧光素进行标记，或用于使用荧光素标记的蛋白A来证明组胺的存在。分离、纯化了超免疫血清的IgG部分，并通过与³H-组胺或含组胺或经组胺处理的细胞结合来检测其特异性。用于检测组胺的组织学标本最好用卡诺伊溶液固定。该程序也可用于组胺的超组织化学检测，使用铁蛋白偶联抗体或过氧化物酶技术。
