通过在膜滤器上进行半连续培养来测定活性腐生水生细菌的数量。
Determination of the number of active saprophytic aquatic bacteria by semi-continuous culture on membrane filters.
作者信息
Kunicka-Goldfinger W, Stronkowska E
出版信息
Acta Microbiol Pol. 1977;26(2):199-205.
The number of bacteria in the hypolimnion waters of Lake Mikpłajski was examined by direct count on membrane filters, by the agar plate method and by semi-continuous culture on membrane filters, as previously proposed by the author. The agar plate method recovered only 20--25% of the bacteria found by direct method. On the other hand, semi-continuous culture on membrane filters allowed the recovery of about 90% of the total count. At the same time it differentiated between active, microcolony forming cells (about 70%) and inactive single cells (about 20% of the total count).
按照作者之前提出的方法，通过膜滤器直接计数、琼脂平板法以及膜滤器半连续培养法，对米克普拉伊斯基湖湖下层水域中的细菌数量进行了检测。琼脂平板法回收的细菌数量仅为直接计数法所发现细菌数量的20%至25%。另一方面，膜滤器半连续培养法能够回收约90%的细菌总数。与此同时，该方法还区分了具有活性的、形成微菌落的细胞（约占总数的70%）和无活性的单细胞（约占总数的20%）。
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