Alton G G, Corner L A, Plackett P
Aust Vet J. 1980 Aug;56(8):369-72. doi: 10.1111/j.1751-0813.1980.tb09561.x.
Two groups, each of 9 Jersey cows, were vaccinated subcutaneously with reduced doses of Brucella abortus strain 19 vaccine (measured by the number of bacteria in the vaccine dose) early in their second pregnancy. Ten weeks later they were challenged, along with a similar group of non-vaccinated cows, by conjunctival instillation of a virulent strain of B. abortus biotype 1. Cows in Group 1 received 1/20th and those in Group 2 received 1/400th the recommended dose of strain 19. Marked reduction in the serological response to vaccination was seen only in Group 2. Four cows in Group 1 excreted strain 19 after parturition, one of them aborted and another calved prematurely with heavy infection of the placenta and foetus with strain 19 in both cases. Resistance to challenge was similar in both vaccinated groups, and higher than previously demonstrated after conventional calfhood vaccination with strain 19. It is concluded that pregnant cows can be effectively vaccinated by the subcutaneous administration of a dose of strain 19 vaccine containing approximately 3 x 10(8) organisms without undue interference with subsequent serological tests or inconvenience resulting from persistence of strain 19 infection.
两组，每组9头泽西奶牛，在其第二次怀孕早期皮下接种减毒剂量的布鲁氏菌19号疫苗（以疫苗剂量中的细菌数量衡量）。十周后，它们与一组未接种疫苗的类似奶牛一起，通过结膜滴注强毒布鲁氏菌生物型1菌株进行攻毒。第1组奶牛接种了1/20推荐剂量的19号菌株，第2组奶牛接种了1/400推荐剂量的19号菌株。仅在第2组中观察到对疫苗接种的血清学反应显著降低。第1组中有4头奶牛在分娩后排出19号菌株，其中1头流产，另一头早产，两种情况下胎盘和胎儿均被19号菌株严重感染。两个接种组对攻毒的抵抗力相似，且高于之前用19号菌株进行常规犊牛期疫苗接种后的抵抗力。得出的结论是，怀孕奶牛皮下接种含有约3×10⁸个菌的19号菌株疫苗剂量可有效接种，且不会对后续血清学检测造成不当干扰，也不会因19号菌株感染持续存在而带来不便。
