Corner L A, Alton G G
Res Vet Sci. 1981 Nov;31(3):342-4.
Brucella abortus strain 19 was recovered at necropsy from cows vaccinated with reduced doses of strain 19, three to six months previously. Strain 19 was isolated from four of nine cows vaccinated with 5.8 x 10(9) viable cells during pregnancy. In two of these nine cows vaccination had led to colonisation of the uterus with erythritol-utilising mutants of strain 19 and consequent abortion or premature calving. In one of another group of nine pregnant vaccinated with 2.8 x 10(8) viable cells, the vaccine strain was recovered from the colostrum. There was no interference with the course of pregnancy in over 700 cattle vaccinated with 3 x 10(8) viable cells. Thirteen of these cows, serologically positive approximately three months after vaccination, were necropsied and strain 19 was isolated from six. This lower dose has already been shown to produce immunity equal to that of higher doses and is recommended as suitable for whole herd vaccination.
在尸检时，从三到六个月前接种过减毒剂量19号菌株的奶牛中分离出了流产布鲁氏菌19号菌株。在怀孕期间接种了5.8×10⁹个活细胞的9头奶牛中，有4头分离出了19号菌株。在这9头奶牛中的2头中，接种导致子宫被利用赤藓醇的19号菌株突变体定植，随后发生流产或早产。在另一组9头怀孕并接种了2.8×10⁸个活细胞的奶牛中，有1头从初乳中分离出了疫苗菌株。在接种3×10⁸个活细胞的700多头奶牛中，怀孕过程未受干扰。这些奶牛中有13头在接种后约三个月血清学呈阳性，对其进行了尸检，其中6头分离出了19号菌株。已证明这种较低剂量产生的免疫力与较高剂量相同，建议将其作为适合全群接种的剂量。
