Mély-Goubert B, Freedman M H
Cancer Biochem Biophys. 1980;4(3):167-71.
Normal human lymphocytes and human tumor cell lines were labeled with an apolar fluorescent probe (1, 6-diphenyl-1, 3, 5-hexatriene), and fluoresence polarization (P) values were determined. The arguments presented in this study suggest that although P values represent an intricate average of all labeled hydrophobic regions of the cell (phospholipid bilayers, membrane proteins and enzymes, etc.), to a large extent, interactions of the probe with membrane proteins are of primary importance. The lower P values obtained with the tumor cell lines, compared with the normal lymphocytes, were interpreted as indicating alterations in either the structure or concentration of a membrane associated protein(s).
用一种非极性荧光探针（1,6 - 二苯基 - 1,3,5 - 己三烯）标记正常人淋巴细胞和人肿瘤细胞系，并测定荧光偏振（P）值。本研究提出的观点表明，虽然P值代表细胞所有标记疏水区域（磷脂双层、膜蛋白和酶等）的复杂平均值，但在很大程度上，探针与膜蛋白的相互作用最为重要。与正常淋巴细胞相比，肿瘤细胞系获得的较低P值被解释为表明膜相关蛋白的结构或浓度发生了改变。
