人类白血病和淋巴瘤细胞中的膜动力学。二苯基己三烯荧光偏振的pH依赖性。

Membrane dynamics in human leukemia and lymphoma cells. pH dependency of diphenylhexatriene fluorescence polarization.

作者信息

Billard C, Phan Dinh Tuy F, Rosenfeld C, Tapiero H

出版信息

FEBS Lett. 1983 Jul 25;158(2):229-33. doi: 10.1016/0014-5793(83)80584-5.

Abstract

Membrane dynamics of human leukemia and lymphoma cell lines were analyzed by investigating the effect of pH on fluorescence polarization (P) of the lipophilic probe diphenylhexatriene (DPH). The degree of P varied as a function of pH, depending on the cell lines. These variations were not detected in phospholipid vesicles. In addition, they were prevented by treatments with glutaraldehyde, sodium azide or phenylmethylsulfanyl fluoride, a specific protease inhibitor. Therefore, these P value changes might be influenced by protein modification.

摘要

通过研究pH值对亲脂性探针二苯基己三烯（DPH）荧光偏振（P）的影响，分析了人类白血病和淋巴瘤细胞系的膜动力学。P的程度随pH值变化，具体取决于细胞系。在磷脂囊泡中未检测到这些变化。此外，用戊二醛、叠氮化钠或特异性蛋白酶抑制剂苯甲基磺酰氟处理可阻止这些变化。因此，这些P值变化可能受蛋白质修饰的影响。