Billard C, Phan Dinh Tuy F, Rosenfeld C, Tapiero H
FEBS Lett. 1983 Jul 25;158(2):229-33. doi: 10.1016/0014-5793(83)80584-5.
Membrane dynamics of human leukemia and lymphoma cell lines were analyzed by investigating the effect of pH on fluorescence polarization (P) of the lipophilic probe diphenylhexatriene (DPH). The degree of P varied as a function of pH, depending on the cell lines. These variations were not detected in phospholipid vesicles. In addition, they were prevented by treatments with glutaraldehyde, sodium azide or phenylmethylsulfanyl fluoride, a specific protease inhibitor. Therefore, these P value changes might be influenced by protein modification.
通过研究pH值对亲脂性探针二苯基己三烯（DPH）荧光偏振（P）的影响，分析了人类白血病和淋巴瘤细胞系的膜动力学。P的程度随pH值变化，具体取决于细胞系。在磷脂囊泡中未检测到这些变化。此外，用戊二醛、叠氮化钠或特异性蛋白酶抑制剂苯甲基磺酰氟处理可阻止这些变化。因此，这些P值变化可能受蛋白质修饰的影响。
