Merritt W D, Morré D J, Doak R L, Keenan T W
Cancer Biochem Biophys. 1980;4(3):183-94.
The specific activities of UDP-galactose : GM2 galactosyltransferase and CMP-NAN : GM1 sialyltransferase were measured in total particulate fractions of individual hepatocellular carcinomas (hepatomas) and hepatic nodules, induced in the rat by a carcinogenic diet of 0.05% N-2-fluorenylacetamide alternating with a low protein basal diet. In all tumors except the smallest nodules, galactosyltransferase specific activities were increased compared to those of control livers from rats fed only basal diet. When relative specific activities were related to wet weight of nodules larger than 0.1 g were in two populations. Specific activities of nodules in one population overlapped those of poorly differentiated hepatomas whereas specific activities of the second population those of well differentiated hepatomas. Specific activities of the sialyltransferase were also increased above those in control livers but not with the same frequency or to the same extent as for the galactosyltransferase. As with galactosyltransferase, nodules were found in two major populations when specific activities were related to nodule wet weight. The data suggest that increased specific activities of galactosyltransferase and sialyltransferase in hepatic nodules may provide an early phenotypic indicator of diverging differentiation in hepatocarcinogenesis.
在由0.05% N-2-芴基乙酰胺致癌饮食与低蛋白基础饮食交替喂养诱导的大鼠个体肝细胞癌（肝癌）和肝结节的总颗粒组分中，测定了UDP-半乳糖：GM2半乳糖基转移酶和CMP-NAN：GM1唾液酸转移酶的比活性。在所有肿瘤中，除了最小的结节外，与仅喂食基础饮食的大鼠的对照肝脏相比，半乳糖基转移酶的比活性增加。当相对比活性与大于0.1 g的结节湿重相关时，结节分为两个群体。一个群体中的结节比活性与低分化肝癌的比活性重叠，而第二个群体中的结节比活性与高分化肝癌的比活性重叠。唾液酸转移酶的比活性也高于对照肝脏中的比活性，但与半乳糖基转移酶的频率或程度不同。与半乳糖基转移酶一样，当比活性与结节湿重相关时，结节分为两个主要群体。数据表明，肝结节中半乳糖基转移酶和唾液酸转移酶比活性的增加可能为肝癌发生过程中分化差异提供早期表型指标。
