Fleischer B
J Supramol Struct. 1977;7(1):79-89. doi: 10.1002/jss.400070108.
Cell fractions from rat kidney were isolated and studied for their ability to synthesize several possible intermediates in the biosynthesis of sulfatides and gangliosides. The enzymes studied include UDP-Gal:ceramide galactosyltransferase, UDP-Gal:glucosylceramide galactosyltransferase, UDP-Gal:galactosylceramide galactosyltransferase, and CMP-NAN:lactosylceramide sialytransferase activities. The initial glycosylation of ceramide was found to be present in all of the kidney cell fractions studied. The remaining glycosylating enzymes were largely localized in the Golgi apparatus of kidney. Thus, in addition to modifying glycoproteins for secretion, the Golgi apparatus in kidney is involved in the modification of a number of glycolipids which are destined to form cell membrane components.
从大鼠肾脏中分离出细胞组分，并研究它们在硫脂和神经节苷脂生物合成中合成几种可能中间体的能力。所研究的酶包括UDP-半乳糖：神经酰胺半乳糖基转移酶、UDP-半乳糖：葡萄糖神经酰胺半乳糖基转移酶、UDP-半乳糖：半乳糖神经酰胺半乳糖基转移酶以及CMP-NAN：乳糖基神经酰胺唾液酸转移酶活性。发现神经酰胺的初始糖基化存在于所有研究的肾脏细胞组分中。其余的糖基化酶主要定位于肾脏的高尔基体中。因此，除了修饰糖蛋白以进行分泌外，肾脏中的高尔基体还参与了许多注定要形成细胞膜成分的糖脂的修饰。
