Schanbacher B D
Int J Androl. 1980 Oct;3(5):563-73. doi: 10.1111/j.1365-2605.1980.tb00144.x.
Testicular and accessory sex gland function was examined in 20 mature breeding rams that received graded dosages of testosterone (T) by subdermal Silastic implants or intramuscular injections. Treatments were imposed for 72 days during the normal breeding season. As compared with control rams rams implanted with 20 T-filled Silastic capsules had greatly reduced testes (24%) and epididymides (53%) weights as well as total daily sperm production (3.3%) and epididymal sperm reserves (3.9%). These effects were produced when serum T concentrations were increased four-fold over those of control rams, serum LH was nondetectable and serum FSH was substantially decreased. Testicular weight and total daily sperm production were less dramatically reduced in rams given T (2 x 250 mg/day) by injection. In spite of the high serum T concentrations observed in injected rams, weights of the accessory sex glands were not significantly affected. Treatment with 5 or 20 T-filled Silastic capsules or by injection of T not only suppressed gonadotropin secretion and spermatogenesis but also reduced T concentrations in rete testis fluid. A high correlation (r = 0.94, P < 0.01) between daily sperm production and T concentrations in rete testis fluid was calculated in this study. Results presented herein indicate that 1) treatment of rams with exogenous T affects spermatogenesis in a dose-dependent manner and 2) spermatogenic yield is related to T concentrations in fluid of the rete testis.
对20只成年种公羊的睾丸及附属性腺功能进行了检测，这些公羊通过皮下硅橡胶植入物或肌肉注射接受了不同剂量的睾酮（T）。在正常繁殖季节进行了72天的处理。与对照公羊相比，植入20个含T硅橡胶胶囊的公羊睾丸重量（降低24%）、附睾重量（降低53%）、每日精子总产量（降低3.3%）和附睾精子储备量（降低3.9%）均大幅下降。这些影响是在血清T浓度比对照公羊增加四倍、血清促黄体生成素（LH）检测不到且血清促卵泡生成素（FSH）大幅降低的情况下产生的。通过注射给予T（2×250毫克/天）的公羊，其睾丸重量和每日精子总产量的降低幅度较小。尽管注射组公羊血清T浓度较高，但其附属性腺重量未受到显著影响。用5个或20个含T硅橡胶胶囊处理或注射T不仅抑制了促性腺激素分泌和精子发生，还降低了睾丸网液中的T浓度。本研究计算得出每日精子产量与睾丸网液中T浓度之间存在高度相关性（r = 0.94，P < 0.01）。本文给出的结果表明：1）用外源性T处理公羊对精子发生有剂量依赖性影响；2）精子发生产量与睾丸网液中的T浓度有关。
