使用由凝集素-抗原复合物诱导产生的特异性抗血清来研究淋病奈瑟菌的外膜抗原。

The use of specific antiserum induced by lectin-antigen complexes to investigate the outer membrane antigens of Neisseria gonorrhoeae.

作者信息

Perera V Y, Penn C W, Smith H

出版信息

J Immunol Methods. 1980;37(2):175-84. doi: 10.1016/0022-1759(80)90202-1.

Abstract

Extracts of gonococcal surface antigens, examined by crossed affinity electrophoresis (CAE) with wheat germ agglutinin (WGA), yielded a single antigen-lectin precipitate in agarose gels. This precipitate induced in rabbits a potent antiserum specific for the gonococcal antigen which reacted with WGA. Immune electron microscopy on whole gonococci showed that this antiserum reacted with outer membrane vesicles. Lipopolysaccharide (LPS) purified by phenol-water extraction of whole gonococci reacted with WGA and also with the antiserum to the WGA-antigen complex. This indicated that the antiserum was specific for antigen(s) of the outer membrane complex.

摘要

用小麦胚凝集素（WGA）通过交叉亲和电泳（CAE）检测的淋球菌表面抗原提取物，在琼脂糖凝胶中产生单一的抗原-凝集素沉淀。这种沉淀在兔体内诱导产生了一种对与WGA反应的淋球菌抗原具有特异性的高效抗血清。对完整淋球菌进行免疫电子显微镜检查表明，这种抗血清与外膜囊泡发生反应。通过酚水提取法从完整淋球菌中纯化的脂多糖（LPS）与WGA反应，也与针对WGA-抗原复合物的抗血清反应。这表明该抗血清对外膜复合物的抗原具有特异性。