受精或激活后海胆卵膜对尿素通透性的变化。
Changes in permeability of sea urchin egg membrane to urea after fertilization or activation.
作者信息
Christen R, Sardet C
出版信息
J Physiol. 1980 Aug;305:1-11. doi: 10.1113/jphysiol.1980.sp013345.
Abstract
- Fertilization or artificial activation of the sea urchin egg, are known to increase the plasma membrane permeability to ions and small metabolites. We have examined the permeability properties of the egg membrane to urea. 2. Urea influx is not saturable and is slightly dependent on temperature. It is inhibited by phloretin. 3. Fertilization leads to a biphasic increase in urea flux kinetics. The first acceleration lasts about 10 min. The second acceleration is maintained at least up to the first cleavage. 4. The ionophore A 23187 mimics exactly fertilization. 5. Weak bases such as procaine lead to a gradual acceleration in urea flux kinetics. The response depends on the dose of procaine added. 6. The inhibitor of oxydative phosphorylation 2,4-DNP fails to block the accelerations triggered by fertilization or activation by A 23187. 7. We discuss the results in light of the early and late events of egg activation.
摘要
- 已知海胆卵的受精或人工激活会增加质膜对离子和小分子代谢物的通透性。我们研究了卵膜对尿素的通透性特性。2. 尿素内流不饱和，且对温度有轻微依赖性。它被根皮素抑制。3. 受精导致尿素通量动力学呈双相增加。第一次加速持续约10分钟。第二次加速至少持续到第一次卵裂。4. 离子载体A 23187能精确模拟受精。5. 弱碱如普鲁卡因会导致尿素通量动力学逐渐加速。反应取决于添加的普鲁卡因剂量。6. 氧化磷酸化抑制剂2,4 -二硝基苯酚不能阻断受精或A 23187激活引发的加速作用。7. 我们根据卵激活的早期和晚期事件来讨论这些结果。
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