Falkner F G, Biessmann H
Nucleic Acids Res. 1980 Mar 11;8(5):943-55. doi: 10.1093/nar/8.5.943.
After 5 minutes heat shock at 37 degrees C Drosophila melanogaster Kc-cell nuclear proteins were extracted wit 0.4M NaCl and compared by SDS gel electrophoresis with extracts from cells grown at 25 degrees C. Two proteins (39 000 and 46 000) were only found in heat shock nuclei. Reconstitution with total Drosophila DNA or a DNA fragment from the heat inducible locus 87A/C covalently coupled to sepharose was performed. In the presence of calf thymus competitor DNA these proteins and also others of lower molecular weight showed preferential binding to the homologous DNA.
在37摄氏度对黑腹果蝇Kc细胞进行5分钟热休克处理后，用0.4M氯化钠提取细胞核蛋白，并通过SDS凝胶电泳与在25摄氏度生长的细胞提取物进行比较。两种蛋白质（39000和46000）仅在热休克细胞核中发现。用果蝇总DNA或来自热诱导位点87A/C的共价偶联到琼脂糖的DNA片段进行了重组。在存在小牛胸腺竞争DNA的情况下，这些蛋白质以及其他分子量较低的蛋白质显示出对同源DNA的优先结合。
