Aber V R, Allen B W, Mitchison D A, Ayuma P, Edwards E A, Keyes A B
Tubercle. 1980 Sep;61(3):123-33. doi: 10.1016/0041-3879(80)90001-x.
Sputum specimens unlikely to contain M. tuberculosis or specimens artificially created as methylcellulose/egg solution were autoclaved and distributed in batches together with sputum specimens containing viable M. tuberculosis marked with an unusual drug-sensitivity pattern. These specimens were prepared in 1 East African laboratory, divided into aliquots and examined by direct smear and culture in 3 East African laboratories. Independent batches were also prepared and cultured in London. Scanty colonies of M. tuberculosis, always with the characteristic marker sensitivity pattern, were obtained from a proportion of the autoclaved specimens, ranging from 0% of 825 specimens in London to 1.3% of 2165 specimens in Kampala. Transfer of bacilli from positive to negative specimens could therefore account for some of the isolated positive cultures that occur in clinical trials. The occurrence of transfer seemed to be related more to the quality of the technician than to the detail of the technical methods. The African laboratories could be graded in their overall efficiency in smear and culture examination.
不太可能含有结核分枝杆菌的痰液标本或人工制成甲基纤维素/鸡蛋溶液的标本经高压灭菌后，与含有具有异常药敏模式标记的活结核分枝杆菌的痰液标本分批分发。这些标本在东非的一个实验室制备，分成小份，在东非的三个实验室进行直接涂片和培养检查。独立的批次也在伦敦制备和培养。从一定比例的高压灭菌标本中获得了少量结核分枝杆菌菌落，其总是具有特征性的标记药敏模式，比例从伦敦825份标本中的0%到坎帕拉2165份标本中的1.3%不等。因此，杆菌从阳性标本转移到阴性标本可能是临床试验中出现一些孤立阳性培养结果的原因。转移的发生似乎更多地与技术人员的素质有关，而不是技术方法的细节。非洲各实验室在涂片和培养检查的总体效率方面可以分级。
