[利用R质粒构建致病性大肠杆菌O112ac、O129、O143血清群及鲍氏志贺菌8型的供体菌株]

[Formation of donor strains of enteropathogenic Escherichia, serogroups O112ac, O129, O143, and Sh. boydii 8 using R-plasmids].

作者信息

Borisov L B, Filippenko O V, Babkov V V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Nov(11):89-92.

Abstract

The possibility of the formation of enteropathogenic Escherichia and Shigella donor strains by means of R-plasmids RP4 and RPI-2 with thermosensitive replication was studied. The donor strains of E. coli, serogroups O112ac, O129, O143, and Sh. boydii 8 were thus obtained; these strains had the above-mentioned plasmids integrated into their chromosome, and in the process of conjugation they transferred plasmid and chromosomal genes to E. coli, K12 and O129, with the effectiveness 10(5)--10(8). Such donor strains may probably be used for studying the mechanisms of the formation of new enteropathogenic Escherichia and Shigella biovars and for their genetic analysis.

摘要

研究了借助具有温度敏感复制特性的R质粒RP4和RPI - 2形成肠道致病性大肠杆菌和志贺氏菌供体菌株的可能性。由此获得了大肠杆菌O112ac、O129、O143血清群以及鲍氏志贺氏菌8型的供体菌株；这些菌株的染色体中整合了上述质粒，并且在接合过程中它们将质粒和染色体基因转移至大肠杆菌K12和O129，转移效率为10(5)--10(8)。这类供体菌株或许可用于研究新型肠道致病性大肠杆菌和志贺氏菌生物变种的形成机制及其遗传分析。