Mise K, Nakajima K, Terakado N, Ishidate M
Gene. 1986;44(1):165-9. doi: 10.1016/0378-1119(86)90058-2.
A convenient procedure has been devised for detection of restriction endonucleases in the Escherichia coli-Shigella group. With this procedure, two restriction endonucleases, designated Sbo 13 and Eco T22, were found and later were identified as isoschizomers of NruI and AvaIII, respectively. These endonucleases were shown to be produced from small multicopy plasmids. They were isolated from nonpathogenic E. coli into which the plasmids had been introduced by transformation, and purified from contaminating nuclease activity. The yield was high, 1,000 units/g of wet cells for Sbo 13 and 500 units/g for Eco T22. Sbo 13 and Eco T22 should be preferable to NruI and AvaIII because of the high yield and ease in handling the producer cells.
已设计出一种简便方法用于检测大肠杆菌-志贺氏菌群中的限制性内切酶。通过该方法，发现了两种限制性内切酶，分别命名为Sbo 13和Eco T22，后来鉴定它们分别是NruI和AvaIII的同裂酶。这些内切酶由小型多拷贝质粒产生。它们从通过转化导入了质粒的非致病性大肠杆菌中分离出来，并从污染的核酸酶活性中纯化得到。产量很高，Sbo 13为每克湿细胞1000单位，Eco T22为每克500单位。由于产量高且处理产生细胞简便，Sbo 13和Eco T22应比NruI和AvaIII更可取。
