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[利用生长抑制试验、补体结合试验和琼脂凝胶扩散沉淀试验鉴定从猴分离出的支原体]

[Use of the growth inhibition test, the CFT, and the agar gel diffusion precipitation test to identify mycoplasmae isolated from monkeys].

作者信息

Efremova I I, Gegenava B O, Kozlova O V, Vasil'eva V I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Aug(8):50-3.

PMID:6778034
Abstract

The data are presented on comparative evaluation of the growth inhibition test (GIT), the diffuse precipitation test in agar gel (DPTAG) and the complement fixation test (CFT) in the identification of Mycoplasma isolated from monkeys. The results obtained in the study of 4 Mycoplasma strains from the internal organs of monkeys in the CFT and the DPTAG with hyperimmune rabbit sera provided by Microbiological Associates, Bethesda, Maryland, USA, allowed these Mycoplasma to be identified as M. laidlawii (l 6916, p 6240 and M 6802) and M. hominis (C 7034), which was confirmed by the study of the biological properties of these strains and in the GIT. The CFT and the DP-TAG, equally to the GIt and other serological tests, can be used for identification of newly isolated Mycoplasma strains.

摘要

本文展示了生长抑制试验(GIT)、琼脂凝胶扩散沉淀试验(DPTAG)和补体结合试验(CFT)在鉴定从猴子分离出的支原体方面的比较评估数据。在美国马里兰州贝塞斯达的微生物学协会提供的超免疫兔血清的帮助下,对从猴子内脏分离出的4株支原体进行CFT和DPTAG研究,所得结果表明这些支原体可被鉴定为莱氏无胆甾原体(16916、p6240和M6802)和人型支原体(C7034),这通过对这些菌株生物学特性的研究以及在GIT中的研究得到了证实。CFT和DPTAG与GIT及其他血清学试验一样,可用于鉴定新分离出的支原体菌株。

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