Hillyer G V, Ramzy R M, El Alamy M A, Cline B L
Am J Trop Med Hyg. 1980 Nov;29(6):1254-7. doi: 10.4269/ajtmh.1980.29.1254.
We utilized the circumoval precipitin (COP) test, Ouchterlony immunodiffusion, and the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human infection with Schistosoma mansoni or S. haematobium, or with both species of schistosome. Only the COP test correctly identified all of those with schistosome infection, although differentiation as to schistosome species was impossible. Circumoval precipitates around S. haematobium eggs from human urine were more numerous and larger than those around S. mansoni eggs obtained from mouse livers. Ouchterlony immunodiffusion with S. mansoni or S. haematobium worm extract failed to diagnose correctly approximately one of every five infected individuals. Mean absorption values of the S. mansoni soluble egg antigen (SEA) used in the ELISA were similar in serum samples from persons infected with either or both species of blood flukes. This suggests that S. mansoni SEA is not species-specific. The ELISA correctly identified, however, 30 of 32 infected individuals, indicating high sensitivity.
我们采用环卵沉淀试验（COP）、双向免疫扩散试验及酶联免疫吸附测定（ELISA）对曼氏血吸虫或埃及血吸虫或两种血吸虫混合感染的人体进行血清学诊断。只有COP试验能正确识别所有血吸虫感染患者，不过无法区分感染的血吸虫种类。人尿中埃及血吸虫虫卵周围的环卵沉淀比小鼠肝脏中曼氏血吸虫虫卵周围的环卵沉淀数量更多、体积更大。用曼氏血吸虫或埃及血吸虫虫体提取物进行双向免疫扩散试验时，约每五名感染者中就有一人无法得到正确诊断。ELISA中所用的曼氏血吸虫可溶性虫卵抗原（SEA）在感染一种或两种血吸虫的人的血清样本中的平均吸收值相似。这表明曼氏血吸虫SEA并非种属特异性的。然而，ELISA正确识别出了32名感染者中的30名，显示出高灵敏度。
