Hillyer G V, Rivera Marrero C
Am J Trop Med Hyg. 1980 Nov;29(6):1249-53. doi: 10.4269/ajtmh.1980.29.1249.
A serum was developed which reacts with Schistosoma mansoni eggs by the circumoval precipitin (COP) test. This antiserum to COP antigens was prepared by coupling the COP immune complexes to protein A-Sepharose, isolating the complex with acid buffer, and immunizing rabbits with the eluate. The antiserum reacted with S. mansoni eggs, forming globular bleb and septate precipitates. It also reacted in gel diffusion with a soluble egg antigen preparation of S. mansoni (SEA). Of four SEA antigenic components precipitated one was clearly MSA1. The antiserum to the COP antigens had anti-parasite antibodies absorbable with lyophilized cercariae or eggs. Three human immunoglobulins (IgG, IgM, and IgA) were involved in the COP reaction.
开发了一种血清，它通过环卵沉淀素（COP）试验与曼氏血吸虫卵发生反应。这种针对COP抗原的抗血清是通过将COP免疫复合物偶联到蛋白 A-琼脂糖上，用酸性缓冲液分离复合物，并用洗脱液免疫兔子制备而成的。该抗血清与曼氏血吸虫卵发生反应，形成球状泡和分隔沉淀。它在凝胶扩散试验中也与曼氏血吸虫的可溶性虫卵抗原制剂（SEA）发生反应。在沉淀的四种SEA抗原成分中，有一种明显是MSA1。针对COP抗原的抗血清具有可被冻干尾蚴或虫卵吸收的抗寄生虫抗体。三种人类免疫球蛋白（IgG、IgM和IgA）参与了COP反应。
