一种新型丙戊酸酶免疫测定法的评估及其与毛细管气相色谱法的比较。

Evaluation of a new valproic acid enzyme immunoassay and comparison with a capillary gas-chromatographic method.

作者信息

Braun S L, Tausch A, Vogt W, Jacob K, Knedel M

出版信息

Clin Chem. 1981 Jan;27(1):169-72.

Abstract

A new homogeneous immunoassay (EMIT) for valproic acid was evaluated. Besides testing the manual version of this enzyme immunoassay, we also developed two mechanized procedures for centrifugal analyzers (the CentrifiChem and the COBAS system), which take less time and are more precise than the manual method. Within-assay precision (CV) was 4.5% with the manual technique and 2% with the analyzers. Between-assay precision (CV) ranged from 4 to 13% for all three techniques. Accuracy of th manual method was checked by dilution and analytical recovery experiments. Our comparison of the EMIT results with those obtained by a comparison method (capillary gas chromatography) showed no significant difference. No interference from hemolysis, hyperbilirubinemia, or aliphatic amino acids was observed. At high concentrations of bile acids and with lipemic sera the analytical recovery rates decreased slightly, to 87% and 92%, respectively.

摘要

对一种新的丙戊酸均相免疫测定法（EMIT）进行了评估。除了测试这种酶免疫测定法的手动版本外，我们还为离心分析仪开发了两种机械化程序（CentrifiChem和COBAS系统），它们比手动方法耗时更少且更精确。手动技术的批内精密度（CV）为4.5%，分析仪的批内精密度（CV）为2%。所有三种技术的批间精密度（CV）在4%至13%之间。通过稀释和分析回收率实验检查了手动方法的准确性。我们将EMIT结果与通过比较方法（毛细管气相色谱法）获得的结果进行比较，未发现显著差异。未观察到溶血、高胆红素血症或脂肪族氨基酸的干扰。在高浓度胆汁酸和脂血血清的情况下，分析回收率分别略有下降，降至87%和92%。