一种用于测定血清中游离糖胺聚糖的半定量微量方法。对不同年龄段健康儿童和黏多糖贮积症患者血清的研究结果。

A semi-quantitative micromethod for the determination of free glycosaminoglycans in serum. Results from studies on serum of healthy children of various age and patients affected by mucopolysaccharidosis.

作者信息

Melet J, Hooghwinkel G J, Giesberts M A, van Gelderen H H

出版信息

Clin Chim Acta. 1980 Dec 8;108(2):179-88. doi: 10.1016/0009-8981(80)90003-0.

Abstract

A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 microliter serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm2; these GAG spots are stained with Alcian Blue. The relationship between the amounts of adsorbed GAG and the optical density of the Alcian Blue spots is linear with a certain range; e.g. for chondroitin sulfate (mixed isomers), from 0.25 to 1.00 micrograms. With this method--which we will refer to as the "DEAE Alcian Blue" method--we estimated the free GAG concentration in sera of individuals of various ages including newborns.

摘要

本文介绍了一种测定低至30微升血清中游离糖胺聚糖（GAG）浓度的方法。通过将血清过滤通过DEAE - 纤维素纸，游离GAG部分被选择性吸附并浓缩在面积为15.9平方毫米的圆形区域上；这些GAG斑点用阿尔辛蓝染色。在一定范围内，吸附的GAG量与阿尔辛蓝斑点的光密度之间呈线性关系；例如，对于硫酸软骨素（混合异构体），范围为0.25至1.00微克。使用这种方法——我们将其称为“DEAE阿尔辛蓝”方法——我们估算了包括新生儿在内的不同年龄段个体血清中的游离GAG浓度。