用阿尔辛蓝8GX对尿液中糖胺聚糖进行定量测定。
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
作者信息
Whiteman P
出版信息
Biochem J. 1973 Feb;131(2):351-7. doi: 10.1042/bj1310351.
Abstract
- The effect of MgCl(2) concentration on the interaction of Alcian Blue 8GX and glycosaminoglycans in the urine of patients with mucopolysaccharidosis was studied by using a new quantitative micro method for the measurement of Alcian Blue-glycosaminoglycan complexes. This provided a means of measuring the critical electrolyte concentrations of urinary glycosaminoglycans. 2. Theoretical considerations based on the preceding paper (Whiteman, 1973) and experimental evidence provided here show that Alcian Blue 8GX may be used for the direct quantitative determination of total urinary glycosaminoglycans. The method is simple, requires sample volumes of 50mul or less, and gives results comparable with those obtained by other more complicated methods.
摘要
- 采用一种新的定量微量方法测定阿尔辛蓝 - 糖胺聚糖复合物，研究了氯化镁浓度对黏多糖贮积症患者尿液中阿尔辛蓝8GX与糖胺聚糖相互作用的影响。这提供了一种测量尿糖胺聚糖临界电解质浓度的方法。2. 基于前文（怀特曼，1973年）的理论思考及此处提供的实验证据表明，阿尔辛蓝8GX可用于直接定量测定尿中总糖胺聚糖。该方法简单，所需样品体积为50微升或更少，且结果与其他更复杂方法所得结果相当。
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