Heinis J J, Beuchat L R, Boswell F C
Appl Environ Microbiol. 1978 Jun;35(6):1035-40. doi: 10.1128/aem.35.6.1035-1040.1978.
Metabolic inhibitors were added to a culture medium inoculated with theramlly stressed Vibrio parahaemolyticus to obtain information pertaining to biosynthetic processes required for recovery from heat damage. Ribonucleic acid and protein syntheses, in addition to membrane repair, were required during recovery of injured cells. Neither nucleic acid nor Mg2+ leakage was noted to occur during the time cells were subjected to heat stress. Studies revealed that Mg2+ was apparently taken up by cells of V. parahaemolyticus during the first 30 min after thermal treatment, indicating a possible increased requirement for Mg2+ for membrane and/or ribosome stability and repair.
将代谢抑制剂添加到接种了热应激副溶血性弧菌的培养基中,以获取与热损伤恢复所需的生物合成过程相关的信息。受损细胞恢复过程中除了需要膜修复外,还需要核糖核酸和蛋白质合成。在细胞遭受热应激期间,未观察到核酸或Mg2+泄漏。研究表明,热处理后的前30分钟内,副溶血性弧菌细胞明显摄取了Mg2+,这表明膜和/或核糖体的稳定性及修复可能对Mg2+的需求量增加。