Shuman H A, Silhavy T J
J Biol Chem. 1981 Jan 25;256(2):560-2.
The malK gene product of Escherichia coli has been identified through the use of a previously described technique that employs gene fusions (Shuman, H. A., Silhavy, T. J., and Beckwith, J. R. (1980) J. Biol. Chem. 255, 168-174). This protein, along with the four other products of the malB locus, comprise the complete maltose transport system. The malK protein has a molecular weight of approximately 40,000 and is located in the cell envelope. In mutant strains which lack another component of the transport system, the malG protein, the malK protein is located in the cytoplasm. This alteration in location suggests that the malK protein is associated with the inner surface of the cytoplasmic membrane via an interaction with the malG protein.
通过使用一种先前描述的利用基因融合的技术（舒曼，H. A.，西尔哈维，T. J.，和贝克威思，J. R.（1980年）《生物化学杂志》255卷，168 - 174页），已鉴定出大肠杆菌的malK基因产物。该蛋白质与malB位点的其他四种产物一起，构成了完整的麦芽糖转运系统。malK蛋白的分子量约为40,000，位于细胞膜中。在缺乏转运系统另一种成分malG蛋白的突变菌株中，malK蛋白位于细胞质中。这种位置的改变表明，malK蛋白通过与malG蛋白的相互作用，与细胞质膜的内表面相关联。
