Albers J J, Adolphson J L, Chen C H
J Clin Invest. 1981 Jan;67(1):141-8. doi: 10.1172/JCI110006.
A sensitive and precise competitive-displacement double-antibody radioimmunoassay was developed for the human plasma enzyme lecithin-cholesterol acyltransferase (LCAT; Ec 2.3 1.43). The ability of plasma from various animal species to displace labeled human LCAT from goat anti-human LCAT could be ranked in the following order: man and sheep > nonhuman primates > cat or dog > pig > rabbit or guinea pig > mouse > rat. Normolipidemic subjects had levels of LCAT of 6.14 +/- 0.98 micrograms/ml (mean +/- SD, n = 66). Subjects with dysbeta-lipoproteinemia had the highest plasma LCAT levels (7.88 +/- 0.39 micrograms/ml, n = 7, P < 0.05), followed by hypercholesterolemic subjects (7.00 +/- 1.30, n = 41) and hypertriglyceridemic subjects (6.96 +/- 1.3, n = 10). LCAT-deficient subjects had the lowest enzyme levels (0.89, 0.83, and 0.05 micrograms/ml, respectively, and two subjects with no detectable enzyme). Males had lower LCAT levels (6.42 +/- 1.05 micrograms/ml, n = 90, for all subjects; 5.99 +/- 1.03, n = 44, for normolipidemics) than females (7.01 +/- 1.14, n = 34, for all subjects P < 0.01; 6.44 +/- 0.79, n = 22, for normolipidemics, P < 0.01). LCAT levels correlated significantly with total cholesterol (males, r = 0.384, P < 0.001; females, r = 0.519, P < 0.002); and total triglyceride (only in females, r = 0.512, P < 0.002). LCAT levels in females correlated inversely with HDL cholesterol (r = 0.341, P < 0.05) and apoprotein D (r = 0.443, P < 0.02), but no such relationship existed in males.
我们开发了一种灵敏且精确的竞争性置换双抗体放射免疫分析法，用于检测人血浆中的卵磷脂胆固醇酰基转移酶（LCAT；EC 2.3 1.43）。不同动物物种的血浆将标记的人LCAT从山羊抗人LCAT中置换出来的能力可按以下顺序排列：人和绵羊＞非人灵长类动物＞猫或狗＞猪＞兔或豚鼠＞小鼠＞大鼠。血脂正常的受试者的LCAT水平为6.14±0.98微克/毫升（平均值±标准差，n = 66）。β脂蛋白异常血症患者的血浆LCAT水平最高（7.88±0.39微克/毫升，n = 7，P＜0.05），其次是高胆固醇血症患者（7.00±1.30，n = 41）和高甘油三酯血症患者（6.96±1.3，n = 10）。LCAT缺乏的受试者的酶水平最低（分别为0.89、0.83和0.05微克/毫升，另有两名受试者未检测到酶）。男性的LCAT水平（所有受试者为6.42±1.05微克/毫升，n = 90；血脂正常者为5.99±1.03，n = 44）低于女性（所有受试者为7.01±1.14，n = 34，P＜0.01；血脂正常者为6.44±0.79，n = 22，P＜0.01）。LCAT水平与总胆固醇显著相关（男性，r = 0.384，P＜0.001；女性，r = 0.519，P＜0.002）；与总甘油三酯也显著相关（仅在女性中，r = 0.512，P＜0.002）。女性的LCAT水平与高密度脂蛋白胆固醇呈负相关（r = 0.341，P＜0.05），与载脂蛋白D呈负相关（r = 0.443，P＜0.02），但男性不存在这种关系。
