Cheng K W, Simaraks S, Palmer W M
J Reprod Fertil. 1981 Jan;61(1):115-21. doi: 10.1530/jrf.0.0610115.
The sensitivity of the assay, utilizing a bovine FSH antiserum and 125I-labelled bovine FSH tracer, is 5 ng NIH-FSH-S6/ml, and cross-reactivities of other ovine pituitary glycoprotein and protein hormones (LH, TSH, growth hormone and prolactin) are less than 1.0%. Both pituitary and serum FSH of the sheep cross-react in a manner parallel with that of standard NIH-FSH-S6. Serum FSH concentrations in 4 ewes at various times after the onset of oestrus peaked more or less coincidently with the preovulatory increase of serum LH. The serum levels of FSH and LH did not correlate proportionally but fluctuated considerably in individuals animals. The radioimmunoassay system appears to be sensitive and specific for measuring serum concentrations of FSH in rams and ewes in different physiological states.
利用牛促卵泡素抗血清和125I标记的牛促卵泡素示踪剂的该检测方法的灵敏度为5纳克NIH - FSH - S6/毫升，其他绵羊垂体糖蛋白和蛋白质激素（促黄体生成素、促甲状腺激素、生长激素和催乳素）的交叉反应率低于1.0%。绵羊的垂体促卵泡素和血清促卵泡素的交叉反应方式与标准NIH - FSH - S6平行。4只母羊在发情开始后不同时间的血清促卵泡素浓度或多或少与排卵前血清促黄体生成素的升高同时达到峰值。促卵泡素和促黄体生成素的血清水平并非成比例相关，而是在个体动物中波动很大。放射免疫分析系统对于测量处于不同生理状态的公羊和母羊血清中促卵泡素的浓度似乎灵敏且特异。
