绵羊促卵泡激素异源放射免疫测定法的开发与应用

Development and application of a heterologous radioimmunoassay for ovine follicle-stimulating hormone.

作者信息

McNeilly J R, McNeilly A S, Walton J S, Cunningham F J

出版信息

J Endocrinol. 1976 Jul;70(1):69-79. doi: 10.1677/joe.0.0700069.

DOI:10.1677/joe.0.0700069

Abstract

A highly specific and sensitive heterologous double antibody radioimmunoassay for ovine follicle-stimulating hormone (oFSH) is described in detail. The assay using a rabbit antiserum to human FSH and either 125I-labelled rat FSH or 125I-labelled oFSH as tracer is specific for FSH. A maximum cross-reaction (B/Bo = 50%) of 0-1% was observed with other ovine, rat or human pituitary hormones or human chorionic gonadotrophin. Serum levels of oFSH in samples collected daily throughout the oestrous cycle showed large individual variations. In five out of nine animals a peak of FSH was observed on the day of oestrus.

摘要

本文详细描述了一种用于检测绵羊促卵泡激素（oFSH）的高特异性和高灵敏度的异源双抗体放射免疫测定法。该测定法使用兔抗人促卵泡激素血清，并以125I标记的大鼠促卵泡激素或125I标记的绵羊促卵泡激素作为示踪剂，对促卵泡激素具有特异性。与其他绵羊、大鼠或人类垂体激素或人绒毛膜促性腺激素的最大交叉反应率（B/Bo = 50%）为0-1%。在整个发情周期中每天采集的样本中，绵羊促卵泡激素的血清水平显示出很大的个体差异。在九只动物中的五只中，在发情当天观察到促卵泡激素峰值。